Dna and sperm fraction
4 Dec Profiling sperm DNA present on vaginal swabs taken from rape victims often In all samples tested, STR profiles of the male DNA fractions.
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Understanding PCR Contamination
The DNA in the separate epithelial cell fraction and the sperm cell fraction can then be isolated by organic or nonorganic methods. This extraction method can.
Description:Among the most useful types of forensic evidence from such cases is the vaginal swab taken from the victim, which often contains sperm, and thus sperm DNA, from the perpetrator. Or, if the sizes overlap, they are tagged with differing dyes to help distinguish the 13 loci. Hot soapy water, a sponge and a round scrub brush are useful for cleaning thermal cyclers and their sample-tube wells. These four letters are short nicknames for more complicated building-block chemical names, but actually the letters A,T, G and C are used much more commonly than the chemical names so the latter will not be mentioned here. Even with fresh samples however, some of the non-sperm DNA will be trapped in the sperm pellet. Now, let's say we want to design a PCR to examine this same locus in other people.